67.7 Homocysteine

67.7.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

Prolactin
Chisq Df Pr(>Chisq)
(Intercept) 250.7924480 1 <0.0001 ***
Study 3.2136720 1 0.07 ns
f39_gdm_new 0.5754347 1 0.4 ns
BMI 3.3368238 1 0.07 ns
Ethnicity_cauc 105.4219556 1 <0.0001 ***
f10c_any_cig_1st_vst 49.1590682 1 <0.0001 ***
GA_samp 164.6323095 1 <0.0001 ***
Study:f39_gdm_new 1.8652579 1 0.2 ns
HPL
Chisq Df Pr(>Chisq)
(Intercept) 150.8759535 1 <0.0001 ***
Study 7.3986291 1 0.007 **
f39_gdm_new 0.4357432 1 0.5 ns
BMI 236.2094019 1 <0.0001 ***
Ethnicity_cauc 5.5165373 1 0.02 *
f10c_any_cig_1st_vst 0.9176455 1 0.3 ns
GA_samp 2601.9722143 1 <0.0001 ***
Study:f39_gdm_new 1.4845286 1 0.2 ns
GH2
Chisq Df Pr(>Chisq)
(Intercept) 251.096669 1 <0.0001 ***
Study 1.107622 1 0.3 ns
f39_gdm_new 1.386104 1 0.2 ns
BMI 87.622496 1 <0.0001 ***
Ethnicity_cauc 3.246832 1 0.07 ns
f10c_any_cig_1st_vst 1.039153 1 0.3 ns
GA_samp 1032.266869 1 <0.0001 ***
Study:f39_gdm_new 3.376951 1 0.07 ns
Insulin
model term df1 df2 F.ratio Chisq p.value
1 Study 1 Inf 21.326 21.326 <0.0001 ***
3 f39_gdm_new 1 Inf 33.726 33.726 <0.0001 ***
4 BMI 1 Inf 330.722 330.722 <0.0001 ***
5 Ethnicity_cauc 1 Inf 25.046 25.046 <0.0001 ***
6 f10c_any_cig_1st_vst 1 Inf 3.158 3.158 0.08 ns
7 GA_samp 1 Inf 2.506 2.506 0.1 ns
2 Study:f39_gdm_new 1 Inf 4.089 4.089 0.04 *
HOMA.IR
model term df1 df2 F.ratio Chisq p.value
1 Study 1 Inf 36.051 36.051 <0.0001 ***
3 f39_gdm_new 1 Inf 32.027 32.027 <0.0001 ***
4 BMI 1 Inf 316.807 316.807 <0.0001 ***
5 Ethnicity_cauc 1 Inf 24.882 24.882 <0.0001 ***
6 f10c_any_cig_1st_vst 1 Inf 2.618 2.618 0.1 ns
7 GA_samp 1 Inf 1.602 1.602 0.2 ns
2 Study:f39_gdm_new 1 Inf 4.361 4.361 0.04 *
GLUC3_mmol.L
model term df1 df2 F.ratio Chisq p.value
1 Study 1 Inf 53.182 53.182 <0.0001 ***
3 f39_gdm_new 1 Inf 4.345 4.345 0.04 *
4 BMI 1 Inf 11.449 11.449 0.0007 ***
5 Ethnicity_cauc 1 Inf 0.718 0.718 0.4 ns
6 f10c_any_cig_1st_vst 1 Inf 0.028 0.028 0.9 ns
7 GA_samp 1 Inf 0.278 0.278 0.6 ns
2 Study:f39_gdm_new 1 Inf 1.558 1.558 0.2 ns

67.7.2 Model summary

Ratio of Geometric means (95% CI)

Prolactin
Var Effect P
(Intercept) 3.11 (2.71, 3.51) <0.0001
Homocysteine -0.06 (-0.10, -0.02) 0.002
f39_gdm_newGDM -0.12 (-0.55, 0.30) 0.57
StudySTOP 0.01 (-0.25, 0.26) 0.95
GA_samp 0.13 (0.11, 0.16) <0.0001
Homocysteine:f39_gdm_newGDM 0.00 (-0.09, 0.09) 0.97
f39_gdm_newGDM:StudySTOP 0.17 (-0.06, 0.40) 0.15
Homocysteine:StudySTOP -0.01 (-0.06, 0.04) 0.79
HPL
Var Effect P
(Intercept) 0.68 (0.47, 0.89) <0.0001
Homocysteine -0.00 (-0.02, 0.02) 0.80
f39_gdm_newGDM 0.20 (-0.02, 0.43) 0.08
StudySTOP 0.14 (0.00, 0.28) 0.05
GA_samp 0.29 (0.28, 0.30) <0.0001
Homocysteine:f39_gdm_newGDM -0.07 (-0.12, -0.02) 0.006
f39_gdm_newGDM:StudySTOP 0.12 (-0.00, 0.24) 0.05
Homocysteine:StudySTOP -0.01 (-0.04, 0.02) 0.47
GH2
Var Effect P
(Intercept) -2.55 (-2.83, -2.26) <0.0001
Homocysteine -0.01 (-0.04, 0.01) 0.34
f39_gdm_newGDM 0.01 (-0.31, 0.33) 0.95
StudySTOP -0.11 (-0.30, 0.08) 0.25
GA_samp 0.26 (0.25, 0.28) <0.0001
Homocysteine:f39_gdm_newGDM -0.04 (-0.11, 0.03) 0.23
f39_gdm_newGDM:StudySTOP 0.21 (0.04, 0.38) 0.02
Homocysteine:StudySTOP 0.02 (-0.02, 0.06) 0.31
Insulin
Var Effect P
(Intercept) 2.89 (2.35, 3.42) <0.0001
Homocysteine -0.09 (-0.15, -0.03) 0.003
f39_gdm_newGDM 2.65 (1.49, 3.81) <0.0001
StudySTOP -0.50 (-0.87, -0.12) 0.009
GA_samp 0.01 (-0.01, 0.04) 0.34
Homocysteine:f39_gdm_newGDM -0.46 (-0.73, -0.19) 0.0009
f39_gdm_newGDM:StudySTOP -1.67 (-3.07, -0.27) 0.02
Homocysteine:StudySTOP 0.05 (-0.02, 0.13) 0.16
Homocysteine:f39_gdm_newGDM:StudySTOP 0.36 (0.05, 0.68) 0.02
HOMA.IR
Var Effect P
(Intercept) 1.53 (0.97, 2.09) <0.0001
Homocysteine -0.10 (-0.16, -0.03) 0.004
f39_gdm_newGDM 3.15 (1.87, 4.42) <0.0001
StudySTOP -0.68 (-1.08, -0.29) 0.0008
GA_samp 0.01 (-0.02, 0.04) 0.48
Homocysteine:f39_gdm_newGDM -0.56 (-0.86, -0.26) 0.0003
f39_gdm_newGDM:StudySTOP -2.09 (-3.61, -0.57) 0.007
Homocysteine:StudySTOP 0.06 (-0.02, 0.14) 0.13
Homocysteine:f39_gdm_newGDM:StudySTOP 0.45 (0.11, 0.80) 0.01
GLUC3_mmol.L
Var Effect P
(Intercept) 1.78 (1.67, 1.88) <0.0001
Homocysteine -0.01 (-0.02, -0.00) 0.04
f39_gdm_newGDM 0.28 (0.07, 0.49) 0.01
StudySTOP -0.21 (-0.29, -0.13) <0.0001
GA_samp -0.00 (-0.01, 0.00) 0.44
Homocysteine:f39_gdm_newGDM -0.04 (-0.08, -0.01) 0.02
f39_gdm_newGDM:StudySTOP -0.04 (-0.14, 0.05) 0.38
Homocysteine:StudySTOP 0.01 (-0.00, 0.03) 0.11

67.7.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Homocysteine concentration (μM) and may be misleading when interaction term is involved.

Prolactin
Study f39_gdm_new response SE df lower.CL upper.CL
SCOPE non-GDM 96.93739 7.296174 88.95225 83.47194 112.5750
STOP non-GDM 94.42804 6.740419 72.19629 81.90403 108.8671
SCOPE GDM 86.29793 11.316786 644.05049 66.70630 111.6436
STOP GDM 99.58158 8.611586 151.48308 83.94141 118.1359
HPL
Study f39_gdm_new response SE df lower.CL upper.CL
SCOPE non-GDM 89.75740 2.434609 165.1749 85.07688 94.69541
STOP non-GDM 98.35183 2.403120 110.6971 93.70319 103.23109
SCOPE GDM 77.80345 4.626732 1028.3059 69.23423 87.43330
STOP GDM 95.78898 3.472769 422.3345 89.20045 102.86414
GH2
Study f39_gdm_new response SE df lower.CL upper.CL
SCOPE non-GDM 2.335576 0.0685072 407.4216 2.204714 2.474207
STOP non-GDM 2.305160 0.0560001 242.9305 2.197450 2.418150
SCOPE GDM 1.911626 0.1586259 1590.5707 1.624489 2.249516
STOP GDM 2.335396 0.1003974 778.5315 2.146401 2.541033
Insulin
Study f39_gdm_new response SE df asymp.LCL asymp.UCL
SCOPE non-GDM 13.76166 0.7009121 Inf 12.45424 15.20633
STOP non-GDM 10.99489 0.4119179 Inf 10.21647 11.83261
SCOPE GDM 19.43257 3.5314927 Inf 13.60946 27.74723
STOP GDM 17.87600 1.3720792 Inf 15.37928 20.77804
HOMA.IR
Study f39_gdm_new response SE df asymp.LCL asymp.UCL
SCOPE non-GDM 3.323180 0.1801687 Inf 2.988170 3.695749
STOP non-GDM 2.290423 0.0903586 Inf 2.119997 2.474549
SCOPE GDM 4.672579 0.9614765 Inf 3.121799 6.993722
STOP GDM 3.846700 0.3156904 Inf 3.275156 4.517983
GLUC3_mmol.L
Study f39_gdm_new response SE df asymp.LCL asymp.UCL
SCOPE non-GDM 5.404288 0.0507698 Inf 5.305692 5.504717
STOP non-GDM 4.704599 0.0347705 Inf 4.636941 4.773244
SCOPE GDM 5.784732 0.2594083 Inf 5.298004 6.316176
STOP GDM 4.818902 0.0617816 Inf 4.699321 4.941526

67.7.4 Comparisons

67.7.4.1 Within Groups

Effect for every 1 unit increase in Homocysteine concentration (μM).
Hormone Contrast Study f39_gdm_new Ratio of Geometric means (95% CI) P-value
Prolactin Homocysteine5.5 / Homocysteine4.5 SCOPE non-GDM 0.943 (0.908, 0.979) 0.002
Prolactin Homocysteine5.5 / Homocysteine4.5 STOP non-GDM 0.936 (0.904, 0.969) 0.0002
Prolactin Homocysteine5.5 / Homocysteine4.5 SCOPE GDM 0.944 (0.858, 1.040) 0.2
Prolactin Homocysteine5.5 / Homocysteine4.5 STOP GDM 0.938 (0.860, 1.022) 0.1
HPL Homocysteine5.5 / Homocysteine4.5 SCOPE non-GDM 0.997 (0.977, 1.018) 0.8
HPL Homocysteine5.5 / Homocysteine4.5 STOP non-GDM 0.987 (0.968, 1.006) 0.2
HPL Homocysteine5.5 / Homocysteine4.5 SCOPE GDM 0.930 (0.883, 0.980) 0.007
HPL Homocysteine5.5 / Homocysteine4.5 STOP GDM 0.921 (0.878, 0.965) 0.0006
GH2 Homocysteine5.5 / Homocysteine4.5 SCOPE non-GDM 0.987 (0.959, 1.015) 0.3
GH2 Homocysteine5.5 / Homocysteine4.5 STOP non-GDM 1.006 (0.980, 1.033) 0.7
GH2 Homocysteine5.5 / Homocysteine4.5 SCOPE GDM 0.946 (0.880, 1.017) 0.1
GH2 Homocysteine5.5 / Homocysteine4.5 STOP GDM 0.964 (0.904, 1.029) 0.3
Insulin Homocysteine5.5 / Homocysteine4.5 SCOPE non-GDM 0.913 (0.860, 0.970) 0.003
Insulin Homocysteine5.5 / Homocysteine4.5 STOP non-GDM 0.964 (0.922, 1.009) 0.1
Insulin Homocysteine5.5 / Homocysteine4.5 SCOPE GDM 0.576 (0.441, 0.751) <0.0001
Insulin Homocysteine5.5 / Homocysteine4.5 STOP GDM 0.874 (0.750, 1.018) 0.08
HOMA.IR Homocysteine5.5 / Homocysteine4.5 SCOPE non-GDM 0.909 (0.852, 0.969) 0.004
HOMA.IR Homocysteine5.5 / Homocysteine4.5 STOP non-GDM 0.968 (0.922, 1.015) 0.2
HOMA.IR Homocysteine5.5 / Homocysteine4.5 SCOPE GDM 0.519 (0.386, 0.697) <0.0001
HOMA.IR Homocysteine5.5 / Homocysteine4.5 STOP GDM 0.869 (0.739, 1.022) 0.09
GLUC3_mmol.L Homocysteine5.5 / Homocysteine4.5 SCOPE non-GDM 0.989 (0.979, 0.999) 0.04
GLUC3_mmol.L Homocysteine5.5 / Homocysteine4.5 STOP non-GDM 1.002 (0.991, 1.014) 0.7
GLUC3_mmol.L Homocysteine5.5 / Homocysteine4.5 SCOPE GDM 0.949 (0.913, 0.986) 0.008
GLUC3_mmol.L Homocysteine5.5 / Homocysteine4.5 STOP GDM 0.962 (0.932, 0.992) 0.01

67.7.4.2 Between Groups

Below are comparisons of the differences in the change in Homocysteine concentration (μM) between groups. I.e. whether the change in Homocysteine concentration (μM) are different between groups.

Prolactin
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 0.9861581 0.0511233 2091.497 0.8908283 1.091689 0.788 ns
STOP/SCOPE (non-GDM) 0.9930549 0.0257404 2091.497 0.9438370 1.044839 0.788 ns
STOP/SCOPE (GDM) 0.9930549 0.0257404 2091.497 0.9438370 1.044839 0.788 ns
GDM/non-GDM (SCOPE) 1.0016420 0.0462236 2092.797 0.9149739 1.096520 0.972 ns
GDM/non-GDM (STOP) 1.0016420 0.0462236 2092.797 0.9149739 1.096520 0.972 ns
HPL
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 0.9797390 0.0278321 2083.436 0.9266499 1.0358696 0.471 ns
STOP/SCOPE (non-GDM) 0.9898177 0.0140592 2083.436 0.9626266 1.0177768 0.471 ns
STOP/SCOPE (GDM) 0.9898177 0.0140592 2083.436 0.9626266 1.0177768 0.471 ns
GDM/non-GDM (SCOPE) 0.9327881 0.0235558 2057.435 0.8877176 0.9801468 0.006 **
GDM/non-GDM (STOP) 0.9327881 0.0235558 2057.435 0.8877176 0.9801468 0.006 **
GH2
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 1.0397700 0.0403462 2148.401 0.9635837 1.121980 0.315 ns
STOP/SCOPE (non-GDM) 1.0196911 0.0197836 2148.401 0.9816230 1.059235 0.315 ns
STOP/SCOPE (GDM) 1.0196911 0.0197836 2148.401 0.9816230 1.059235 0.315 ns
GDM/non-GDM (SCOPE) 0.9587366 0.0333350 2148.890 0.8955433 1.026389 0.226 ns
GDM/non-GDM (STOP) 0.9587366 0.0333350 2148.890 0.8955433 1.026389 0.226 ns
Insulin
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.6020617 0.2577237 Inf 1.1688167 2.1958974 0.003 **
STOP/SCOPE (non-GDM) 1.0560852 0.0405808 Inf 0.9794696 1.1386939 0.156 ns
STOP/SCOPE (GDM) 1.5169814 0.2371161 Inf 1.1166852 2.0607710 0.008 **
GDM/non-GDM (SCOPE) 0.6306635 0.0877335 Inf 0.4801584 0.8283442 0.0009 ***
GDM/non-GDM (STOP) 0.9058973 0.0734865 Inf 0.7727329 1.0620098 0.223 ns
HOMA.IR
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.7829760 0.3143551 Inf 1.2620333 2.5189536 0.001 **
STOP/SCOPE (non-GDM) 1.0645212 0.0438052 Inf 0.9820356 1.1539351 0.129 ns
STOP/SCOPE (GDM) 1.6749089 0.2871760 Inf 1.1968677 2.3438847 0.003 **
GDM/non-GDM (SCOPE) 0.5707085 0.0878613 Inf 0.4220566 0.7717168 0.0003 ***
GDM/non-GDM (STOP) 0.8979480 0.0772057 Inf 0.7586907 1.0627661 0.211 ns
GLUC3_mmol.L
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.0274009 0.0171900 Inf 0.9942555 1.0616512 0.106 ns
STOP/SCOPE (non-GDM) 1.0136079 0.0084796 Inf 0.9971236 1.0303646 0.106 ns
STOP/SCOPE (GDM) 1.0136079 0.0084796 Inf 0.9971236 1.0303646 0.106 ns
GDM/non-GDM (SCOPE) 0.9593689 0.0171755 Inf 0.9262892 0.9936299 0.021 *
GDM/non-GDM (STOP) 0.9593689 0.0171755 Inf 0.9262892 0.9936299 0.021 *

67.7.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

67.7.6 Plots