67.7 Homocysteine
67.7.1 Global tests
Type III ANOVA on main and interaction effects, and LM model summaries.
Prolactin| Chisq | Df | Pr(>Chisq) | ||
|---|---|---|---|---|
| (Intercept) | 250.7924480 | 1 | <0.0001 | *** |
| Study | 3.2136720 | 1 | 0.07 | ns |
| f39_gdm_new | 0.5754347 | 1 | 0.4 | ns |
| BMI | 3.3368238 | 1 | 0.07 | ns |
| Ethnicity_cauc | 105.4219556 | 1 | <0.0001 | *** |
| f10c_any_cig_1st_vst | 49.1590682 | 1 | <0.0001 | *** |
| GA_samp | 164.6323095 | 1 | <0.0001 | *** |
| Study:f39_gdm_new | 1.8652579 | 1 | 0.2 | ns |
| Chisq | Df | Pr(>Chisq) | ||
|---|---|---|---|---|
| (Intercept) | 150.8759535 | 1 | <0.0001 | *** |
| Study | 7.3986291 | 1 | 0.007 | ** |
| f39_gdm_new | 0.4357432 | 1 | 0.5 | ns |
| BMI | 236.2094019 | 1 | <0.0001 | *** |
| Ethnicity_cauc | 5.5165373 | 1 | 0.02 | * |
| f10c_any_cig_1st_vst | 0.9176455 | 1 | 0.3 | ns |
| GA_samp | 2601.9722143 | 1 | <0.0001 | *** |
| Study:f39_gdm_new | 1.4845286 | 1 | 0.2 | ns |
| Chisq | Df | Pr(>Chisq) | ||
|---|---|---|---|---|
| (Intercept) | 251.096669 | 1 | <0.0001 | *** |
| Study | 1.107622 | 1 | 0.3 | ns |
| f39_gdm_new | 1.386104 | 1 | 0.2 | ns |
| BMI | 87.622496 | 1 | <0.0001 | *** |
| Ethnicity_cauc | 3.246832 | 1 | 0.07 | ns |
| f10c_any_cig_1st_vst | 1.039153 | 1 | 0.3 | ns |
| GA_samp | 1032.266869 | 1 | <0.0001 | *** |
| Study:f39_gdm_new | 3.376951 | 1 | 0.07 | ns |
| model term | df1 | df2 | F.ratio | Chisq | p.value | ||
|---|---|---|---|---|---|---|---|
| 1 | Study | 1 | Inf | 21.326 | 21.326 | <0.0001 | *** |
| 3 | f39_gdm_new | 1 | Inf | 33.726 | 33.726 | <0.0001 | *** |
| 4 | BMI | 1 | Inf | 330.722 | 330.722 | <0.0001 | *** |
| 5 | Ethnicity_cauc | 1 | Inf | 25.046 | 25.046 | <0.0001 | *** |
| 6 | f10c_any_cig_1st_vst | 1 | Inf | 3.158 | 3.158 | 0.08 | ns |
| 7 | GA_samp | 1 | Inf | 2.506 | 2.506 | 0.1 | ns |
| 2 | Study:f39_gdm_new | 1 | Inf | 4.089 | 4.089 | 0.04 | * |
| model term | df1 | df2 | F.ratio | Chisq | p.value | ||
|---|---|---|---|---|---|---|---|
| 1 | Study | 1 | Inf | 36.051 | 36.051 | <0.0001 | *** |
| 3 | f39_gdm_new | 1 | Inf | 32.027 | 32.027 | <0.0001 | *** |
| 4 | BMI | 1 | Inf | 316.807 | 316.807 | <0.0001 | *** |
| 5 | Ethnicity_cauc | 1 | Inf | 24.882 | 24.882 | <0.0001 | *** |
| 6 | f10c_any_cig_1st_vst | 1 | Inf | 2.618 | 2.618 | 0.1 | ns |
| 7 | GA_samp | 1 | Inf | 1.602 | 1.602 | 0.2 | ns |
| 2 | Study:f39_gdm_new | 1 | Inf | 4.361 | 4.361 | 0.04 | * |
| model term | df1 | df2 | F.ratio | Chisq | p.value | ||
|---|---|---|---|---|---|---|---|
| 1 | Study | 1 | Inf | 53.182 | 53.182 | <0.0001 | *** |
| 3 | f39_gdm_new | 1 | Inf | 4.345 | 4.345 | 0.04 | * |
| 4 | BMI | 1 | Inf | 11.449 | 11.449 | 0.0007 | *** |
| 5 | Ethnicity_cauc | 1 | Inf | 0.718 | 0.718 | 0.4 | ns |
| 6 | f10c_any_cig_1st_vst | 1 | Inf | 0.028 | 0.028 | 0.9 | ns |
| 7 | GA_samp | 1 | Inf | 0.278 | 0.278 | 0.6 | ns |
| 2 | Study:f39_gdm_new | 1 | Inf | 1.558 | 1.558 | 0.2 | ns |
67.7.2 Model summary
Ratio of Geometric means (95% CI)
Prolactin| Var | Effect | P |
|---|---|---|
| (Intercept) | 3.11 (2.71, 3.51) | <0.0001 |
| Homocysteine | -0.06 (-0.10, -0.02) | 0.002 |
| f39_gdm_newGDM | -0.12 (-0.55, 0.30) | 0.57 |
| StudySTOP | 0.01 (-0.25, 0.26) | 0.95 |
| GA_samp | 0.13 (0.11, 0.16) | <0.0001 |
| Homocysteine:f39_gdm_newGDM | 0.00 (-0.09, 0.09) | 0.97 |
| f39_gdm_newGDM:StudySTOP | 0.17 (-0.06, 0.40) | 0.15 |
| Homocysteine:StudySTOP | -0.01 (-0.06, 0.04) | 0.79 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 0.68 (0.47, 0.89) | <0.0001 |
| Homocysteine | -0.00 (-0.02, 0.02) | 0.80 |
| f39_gdm_newGDM | 0.20 (-0.02, 0.43) | 0.08 |
| StudySTOP | 0.14 (0.00, 0.28) | 0.05 |
| GA_samp | 0.29 (0.28, 0.30) | <0.0001 |
| Homocysteine:f39_gdm_newGDM | -0.07 (-0.12, -0.02) | 0.006 |
| f39_gdm_newGDM:StudySTOP | 0.12 (-0.00, 0.24) | 0.05 |
| Homocysteine:StudySTOP | -0.01 (-0.04, 0.02) | 0.47 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | -2.55 (-2.83, -2.26) | <0.0001 |
| Homocysteine | -0.01 (-0.04, 0.01) | 0.34 |
| f39_gdm_newGDM | 0.01 (-0.31, 0.33) | 0.95 |
| StudySTOP | -0.11 (-0.30, 0.08) | 0.25 |
| GA_samp | 0.26 (0.25, 0.28) | <0.0001 |
| Homocysteine:f39_gdm_newGDM | -0.04 (-0.11, 0.03) | 0.23 |
| f39_gdm_newGDM:StudySTOP | 0.21 (0.04, 0.38) | 0.02 |
| Homocysteine:StudySTOP | 0.02 (-0.02, 0.06) | 0.31 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 2.89 (2.35, 3.42) | <0.0001 |
| Homocysteine | -0.09 (-0.15, -0.03) | 0.003 |
| f39_gdm_newGDM | 2.65 (1.49, 3.81) | <0.0001 |
| StudySTOP | -0.50 (-0.87, -0.12) | 0.009 |
| GA_samp | 0.01 (-0.01, 0.04) | 0.34 |
| Homocysteine:f39_gdm_newGDM | -0.46 (-0.73, -0.19) | 0.0009 |
| f39_gdm_newGDM:StudySTOP | -1.67 (-3.07, -0.27) | 0.02 |
| Homocysteine:StudySTOP | 0.05 (-0.02, 0.13) | 0.16 |
| Homocysteine:f39_gdm_newGDM:StudySTOP | 0.36 (0.05, 0.68) | 0.02 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 1.53 (0.97, 2.09) | <0.0001 |
| Homocysteine | -0.10 (-0.16, -0.03) | 0.004 |
| f39_gdm_newGDM | 3.15 (1.87, 4.42) | <0.0001 |
| StudySTOP | -0.68 (-1.08, -0.29) | 0.0008 |
| GA_samp | 0.01 (-0.02, 0.04) | 0.48 |
| Homocysteine:f39_gdm_newGDM | -0.56 (-0.86, -0.26) | 0.0003 |
| f39_gdm_newGDM:StudySTOP | -2.09 (-3.61, -0.57) | 0.007 |
| Homocysteine:StudySTOP | 0.06 (-0.02, 0.14) | 0.13 |
| Homocysteine:f39_gdm_newGDM:StudySTOP | 0.45 (0.11, 0.80) | 0.01 |
| Var | Effect | P |
|---|---|---|
| (Intercept) | 1.78 (1.67, 1.88) | <0.0001 |
| Homocysteine | -0.01 (-0.02, -0.00) | 0.04 |
| f39_gdm_newGDM | 0.28 (0.07, 0.49) | 0.01 |
| StudySTOP | -0.21 (-0.29, -0.13) | <0.0001 |
| GA_samp | -0.00 (-0.01, 0.00) | 0.44 |
| Homocysteine:f39_gdm_newGDM | -0.04 (-0.08, -0.01) | 0.02 |
| f39_gdm_newGDM:StudySTOP | -0.04 (-0.14, 0.05) | 0.38 |
| Homocysteine:StudySTOP | 0.01 (-0.00, 0.03) | 0.11 |
67.7.3 Estimated means
Estimated marginal means (or adjusted means) adjusted for GA at sampling.
Results are averaged over the levels of Homocysteine concentration (μM) and may be misleading when interaction term is involved.
| Study | f39_gdm_new | response | SE | df | lower.CL | upper.CL |
|---|---|---|---|---|---|---|
| SCOPE | non-GDM | 96.93739 | 7.296174 | 88.95225 | 83.47194 | 112.5750 |
| STOP | non-GDM | 94.42804 | 6.740419 | 72.19629 | 81.90403 | 108.8671 |
| SCOPE | GDM | 86.29793 | 11.316786 | 644.05049 | 66.70630 | 111.6436 |
| STOP | GDM | 99.58158 | 8.611586 | 151.48308 | 83.94141 | 118.1359 |
| Study | f39_gdm_new | response | SE | df | lower.CL | upper.CL |
|---|---|---|---|---|---|---|
| SCOPE | non-GDM | 89.75740 | 2.434609 | 165.1749 | 85.07688 | 94.69541 |
| STOP | non-GDM | 98.35183 | 2.403120 | 110.6971 | 93.70319 | 103.23109 |
| SCOPE | GDM | 77.80345 | 4.626732 | 1028.3059 | 69.23423 | 87.43330 |
| STOP | GDM | 95.78898 | 3.472769 | 422.3345 | 89.20045 | 102.86414 |
| Study | f39_gdm_new | response | SE | df | lower.CL | upper.CL |
|---|---|---|---|---|---|---|
| SCOPE | non-GDM | 2.335576 | 0.0685072 | 407.4216 | 2.204714 | 2.474207 |
| STOP | non-GDM | 2.305160 | 0.0560001 | 242.9305 | 2.197450 | 2.418150 |
| SCOPE | GDM | 1.911626 | 0.1586259 | 1590.5707 | 1.624489 | 2.249516 |
| STOP | GDM | 2.335396 | 0.1003974 | 778.5315 | 2.146401 | 2.541033 |
| Study | f39_gdm_new | response | SE | df | asymp.LCL | asymp.UCL |
|---|---|---|---|---|---|---|
| SCOPE | non-GDM | 13.76166 | 0.7009121 | Inf | 12.45424 | 15.20633 |
| STOP | non-GDM | 10.99489 | 0.4119179 | Inf | 10.21647 | 11.83261 |
| SCOPE | GDM | 19.43257 | 3.5314927 | Inf | 13.60946 | 27.74723 |
| STOP | GDM | 17.87600 | 1.3720792 | Inf | 15.37928 | 20.77804 |
| Study | f39_gdm_new | response | SE | df | asymp.LCL | asymp.UCL |
|---|---|---|---|---|---|---|
| SCOPE | non-GDM | 3.323180 | 0.1801687 | Inf | 2.988170 | 3.695749 |
| STOP | non-GDM | 2.290423 | 0.0903586 | Inf | 2.119997 | 2.474549 |
| SCOPE | GDM | 4.672579 | 0.9614765 | Inf | 3.121799 | 6.993722 |
| STOP | GDM | 3.846700 | 0.3156904 | Inf | 3.275156 | 4.517983 |
| Study | f39_gdm_new | response | SE | df | asymp.LCL | asymp.UCL |
|---|---|---|---|---|---|---|
| SCOPE | non-GDM | 5.404288 | 0.0507698 | Inf | 5.305692 | 5.504717 |
| STOP | non-GDM | 4.704599 | 0.0347705 | Inf | 4.636941 | 4.773244 |
| SCOPE | GDM | 5.784732 | 0.2594083 | Inf | 5.298004 | 6.316176 |
| STOP | GDM | 4.818902 | 0.0617816 | Inf | 4.699321 | 4.941526 |
67.7.4 Comparisons
67.7.4.1 Within Groups
Effect for every 1 unit increase in Homocysteine concentration (μM).| Hormone | Contrast | Study | f39_gdm_new | Ratio of Geometric means (95% CI) | P-value |
|---|---|---|---|---|---|
| Prolactin | Homocysteine5.5 / Homocysteine4.5 | SCOPE | non-GDM | 0.943 (0.908, 0.979) | 0.002 |
| Prolactin | Homocysteine5.5 / Homocysteine4.5 | STOP | non-GDM | 0.936 (0.904, 0.969) | 0.0002 |
| Prolactin | Homocysteine5.5 / Homocysteine4.5 | SCOPE | GDM | 0.944 (0.858, 1.040) | 0.2 |
| Prolactin | Homocysteine5.5 / Homocysteine4.5 | STOP | GDM | 0.938 (0.860, 1.022) | 0.1 |
| HPL | Homocysteine5.5 / Homocysteine4.5 | SCOPE | non-GDM | 0.997 (0.977, 1.018) | 0.8 |
| HPL | Homocysteine5.5 / Homocysteine4.5 | STOP | non-GDM | 0.987 (0.968, 1.006) | 0.2 |
| HPL | Homocysteine5.5 / Homocysteine4.5 | SCOPE | GDM | 0.930 (0.883, 0.980) | 0.007 |
| HPL | Homocysteine5.5 / Homocysteine4.5 | STOP | GDM | 0.921 (0.878, 0.965) | 0.0006 |
| GH2 | Homocysteine5.5 / Homocysteine4.5 | SCOPE | non-GDM | 0.987 (0.959, 1.015) | 0.3 |
| GH2 | Homocysteine5.5 / Homocysteine4.5 | STOP | non-GDM | 1.006 (0.980, 1.033) | 0.7 |
| GH2 | Homocysteine5.5 / Homocysteine4.5 | SCOPE | GDM | 0.946 (0.880, 1.017) | 0.1 |
| GH2 | Homocysteine5.5 / Homocysteine4.5 | STOP | GDM | 0.964 (0.904, 1.029) | 0.3 |
| Insulin | Homocysteine5.5 / Homocysteine4.5 | SCOPE | non-GDM | 0.913 (0.860, 0.970) | 0.003 |
| Insulin | Homocysteine5.5 / Homocysteine4.5 | STOP | non-GDM | 0.964 (0.922, 1.009) | 0.1 |
| Insulin | Homocysteine5.5 / Homocysteine4.5 | SCOPE | GDM | 0.576 (0.441, 0.751) | <0.0001 |
| Insulin | Homocysteine5.5 / Homocysteine4.5 | STOP | GDM | 0.874 (0.750, 1.018) | 0.08 |
| HOMA.IR | Homocysteine5.5 / Homocysteine4.5 | SCOPE | non-GDM | 0.909 (0.852, 0.969) | 0.004 |
| HOMA.IR | Homocysteine5.5 / Homocysteine4.5 | STOP | non-GDM | 0.968 (0.922, 1.015) | 0.2 |
| HOMA.IR | Homocysteine5.5 / Homocysteine4.5 | SCOPE | GDM | 0.519 (0.386, 0.697) | <0.0001 |
| HOMA.IR | Homocysteine5.5 / Homocysteine4.5 | STOP | GDM | 0.869 (0.739, 1.022) | 0.09 |
| GLUC3_mmol.L | Homocysteine5.5 / Homocysteine4.5 | SCOPE | non-GDM | 0.989 (0.979, 0.999) | 0.04 |
| GLUC3_mmol.L | Homocysteine5.5 / Homocysteine4.5 | STOP | non-GDM | 1.002 (0.991, 1.014) | 0.7 |
| GLUC3_mmol.L | Homocysteine5.5 / Homocysteine4.5 | SCOPE | GDM | 0.949 (0.913, 0.986) | 0.008 |
| GLUC3_mmol.L | Homocysteine5.5 / Homocysteine4.5 | STOP | GDM | 0.962 (0.932, 0.992) | 0.01 |
67.7.4.2 Between Groups
Below are comparisons of the differences in the change in Homocysteine concentration (μM) between groups. I.e. whether the change in Homocysteine concentration (μM) are different between groups.
| contrast | ratio | SE | df | lower.CL | upper.CL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 0.9861581 | 0.0511233 | 2091.497 | 0.8908283 | 1.091689 | 0.788 | ns |
| STOP/SCOPE (non-GDM) | 0.9930549 | 0.0257404 | 2091.497 | 0.9438370 | 1.044839 | 0.788 | ns |
| STOP/SCOPE (GDM) | 0.9930549 | 0.0257404 | 2091.497 | 0.9438370 | 1.044839 | 0.788 | ns |
| GDM/non-GDM (SCOPE) | 1.0016420 | 0.0462236 | 2092.797 | 0.9149739 | 1.096520 | 0.972 | ns |
| GDM/non-GDM (STOP) | 1.0016420 | 0.0462236 | 2092.797 | 0.9149739 | 1.096520 | 0.972 | ns |
| contrast | ratio | SE | df | lower.CL | upper.CL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 0.9797390 | 0.0278321 | 2083.436 | 0.9266499 | 1.0358696 | 0.471 | ns |
| STOP/SCOPE (non-GDM) | 0.9898177 | 0.0140592 | 2083.436 | 0.9626266 | 1.0177768 | 0.471 | ns |
| STOP/SCOPE (GDM) | 0.9898177 | 0.0140592 | 2083.436 | 0.9626266 | 1.0177768 | 0.471 | ns |
| GDM/non-GDM (SCOPE) | 0.9327881 | 0.0235558 | 2057.435 | 0.8877176 | 0.9801468 | 0.006 | ** |
| GDM/non-GDM (STOP) | 0.9327881 | 0.0235558 | 2057.435 | 0.8877176 | 0.9801468 | 0.006 | ** |
| contrast | ratio | SE | df | lower.CL | upper.CL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.0397700 | 0.0403462 | 2148.401 | 0.9635837 | 1.121980 | 0.315 | ns |
| STOP/SCOPE (non-GDM) | 1.0196911 | 0.0197836 | 2148.401 | 0.9816230 | 1.059235 | 0.315 | ns |
| STOP/SCOPE (GDM) | 1.0196911 | 0.0197836 | 2148.401 | 0.9816230 | 1.059235 | 0.315 | ns |
| GDM/non-GDM (SCOPE) | 0.9587366 | 0.0333350 | 2148.890 | 0.8955433 | 1.026389 | 0.226 | ns |
| GDM/non-GDM (STOP) | 0.9587366 | 0.0333350 | 2148.890 | 0.8955433 | 1.026389 | 0.226 | ns |
| contrast | ratio | SE | df | asymp.LCL | asymp.UCL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.6020617 | 0.2577237 | Inf | 1.1688167 | 2.1958974 | 0.003 | ** |
| STOP/SCOPE (non-GDM) | 1.0560852 | 0.0405808 | Inf | 0.9794696 | 1.1386939 | 0.156 | ns |
| STOP/SCOPE (GDM) | 1.5169814 | 0.2371161 | Inf | 1.1166852 | 2.0607710 | 0.008 | ** |
| GDM/non-GDM (SCOPE) | 0.6306635 | 0.0877335 | Inf | 0.4801584 | 0.8283442 | 0.0009 | *** |
| GDM/non-GDM (STOP) | 0.9058973 | 0.0734865 | Inf | 0.7727329 | 1.0620098 | 0.223 | ns |
| contrast | ratio | SE | df | asymp.LCL | asymp.UCL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.7829760 | 0.3143551 | Inf | 1.2620333 | 2.5189536 | 0.001 | ** |
| STOP/SCOPE (non-GDM) | 1.0645212 | 0.0438052 | Inf | 0.9820356 | 1.1539351 | 0.129 | ns |
| STOP/SCOPE (GDM) | 1.6749089 | 0.2871760 | Inf | 1.1968677 | 2.3438847 | 0.003 | ** |
| GDM/non-GDM (SCOPE) | 0.5707085 | 0.0878613 | Inf | 0.4220566 | 0.7717168 | 0.0003 | *** |
| GDM/non-GDM (STOP) | 0.8979480 | 0.0772057 | Inf | 0.7586907 | 1.0627661 | 0.211 | ns |
| contrast | ratio | SE | df | asymp.LCL | asymp.UCL | p.value | |
|---|---|---|---|---|---|---|---|
| SCOPE/SCOPE (Overall) | 1.0274009 | 0.0171900 | Inf | 0.9942555 | 1.0616512 | 0.106 | ns |
| STOP/SCOPE (non-GDM) | 1.0136079 | 0.0084796 | Inf | 0.9971236 | 1.0303646 | 0.106 | ns |
| STOP/SCOPE (GDM) | 1.0136079 | 0.0084796 | Inf | 0.9971236 | 1.0303646 | 0.106 | ns |
| GDM/non-GDM (SCOPE) | 0.9593689 | 0.0171755 | Inf | 0.9262892 | 0.9936299 | 0.021 | * |
| GDM/non-GDM (STOP) | 0.9593689 | 0.0171755 | Inf | 0.9262892 | 0.9936299 | 0.021 | * |